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Becton Dickinson express hyb hybridization solution
Express Hyb Hybridization Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/express+hyb+hybridization+solution/express+hyb+hybridization+solution/pmc03087329-99-5-9
Average 90 stars, based on 1 article reviews
express hyb hybridization solution - by Bioz Stars, 2026-11
90/100 stars

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Polymerase Chain Reaction:

Article Title: The Bax/Bak ortholog in Drosophila , Debcl, exerts limited control over programmed cell death
Article Snippet: .. The membranes were probed with [ 32 P]dCTP-labeled DNA from PCR product using primers 5′-GCT ACA GTC GAG TGT GCT GGG TTG TTT GCG-3′ and 5′-GAC GGC GGA TTC CAG ACG CTT TCA GAA CGG-3′ for verification of the left recombination arm, and primers 5′-GCG TAC AAT TAG ACC AGC CGT TGT GTT GGC-3′ and 5′-AAG AGG ACA ACA GCG AGG TGG AGG AGG ACG-3′ for verification of the right recombination arm, and hybridized using Express Hyb Hybridization Solution (BD Biosciences). fig ft0 fig mode=article f1 Fig. 1. caption a4 Generation and verification of a targeted debcl mutation. ( A ) Targeting scheme for the debcl gene. .. Southern blotting For verification of targeting, genomic DNA was prepared from flies carrying the candidate targeted allele using the Wizard Genomic Purification Kit (Promega).

Hybridization:

Article Title: The Bax/Bak ortholog in Drosophila , Debcl, exerts limited control over programmed cell death
Article Snippet: .. The membranes were probed with [ 32 P]dCTP-labeled DNA from PCR product using primers 5′-GCT ACA GTC GAG TGT GCT GGG TTG TTT GCG-3′ and 5′-GAC GGC GGA TTC CAG ACG CTT TCA GAA CGG-3′ for verification of the left recombination arm, and primers 5′-GCG TAC AAT TAG ACC AGC CGT TGT GTT GGC-3′ and 5′-AAG AGG ACA ACA GCG AGG TGG AGG AGG ACG-3′ for verification of the right recombination arm, and hybridized using Express Hyb Hybridization Solution (BD Biosciences). fig ft0 fig mode=article f1 Fig. 1. caption a4 Generation and verification of a targeted debcl mutation. ( A ) Targeting scheme for the debcl gene. .. Southern blotting For verification of targeting, genomic DNA was prepared from flies carrying the candidate targeted allele using the Wizard Genomic Purification Kit (Promega).

Article Title: Simultaneous detection of multiple mRNA markers CK19, CEA, c-Met, Her2/neu and hMAM with membrane array, an innovative technique with a great potential for breast cancer diagnosis.
Article Snippet: The objective of this study was mainly to develop and evaluate a membrane array-based method simultaneously detecting the expression levels of a multiple mRNAmarker panel in the peripheral blood for used in complementary breast cancer diagnosis.. The mRNA markers employed included cytokeratin 19 (CK-19), carcinoembryonic antigen (CEA), c-Met, Her2/neu, and mammaglobin (hMAM).. The specimens of peripheral blood were collected from 80 healthy women and 102 female patients with breast cancer.

Article Title: Inhibition of nitric oxide production by the carbazole compound LCY-2-CHO via blockade of activator protein-1 and CCAAT/enhancer-binding protein activation in microglia.
Article Snippet: Excessive nitric oxide (NO) production by activated microglia plays a critical rodegenerative disorders.. In this study, we found that 9-(2-chlorobenyl)-9H carbaldehyde (LCY-2-CHO) suppressed the NO production in lipopolysaccharid feron-g (IFNg)-stimulated murine microglial N9 and BV-2 cells and in LPS-st cells and rat primary microglia.. LCY-2-CHO had no cytotoxic effect on microglia N9 cells, LCY-2-CHO abolished the expression of inducible nitric oxide syn protein and mRNA but failed to alter the stability of expressed iNOS mR enzymatic activity of expressed iNOS protein.

Article Title: Differential gene expression profile of MAGE family in taiwanese patients with colorectal cancer.
Article Snippet: FU-YEN CHUNG, PhD, TIAN-LU CHENG, PhD, HUI-JEN CHANG, MD, HUA-HSIEN CHIU, PhD, MING-YII HUANG, MD, PhD, MING-SUNG CHANG, MD, CHUNG-CHI CHEN, PhD, MING-JE YANG, PhD, JAW-YUAN WANG, MD, PhD,** AND SHIU-RU LIN, PhD* Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Faculty of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan School of Medical and Health Sciences, Fooyin University, Kaohsiung County, Taiwan Department of Medical Research, Fooyin University Hospital, Pingtung County, Taiwan School of Environmental and Life Sciences, Fooyin University, Kaohsiung County, Taiwan Research and Development Headquarters, Fooyin University, Kaohsiung County, Taiwan Division of Genomic Medicine, Fooyin University Hospital, Pingtung County, Taiwan Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Department of Surgery, Fooyin University Hospital, Pingtung County, Taiwan Department of Medical Technology, School of Medical and Health Sciences, Fooyin University, Kaohsiung County, Taiwan Cancer Center, and Division of Gastrointestinal and General Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Department of Medical Research, Fooyin University Hospital, Pingtung County, Taiwan

Article Title: Overexpression of EGFR pathway-related genes in the circulation is highly correlated with EGFR mutations and overexpression in paired cancer tissue from patients with non-small cell lung cancer
Article Snippet: .. The lifts were covered with the Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA, USA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphatase-conjugated antidigoxigenin antibody (Roche Diagnostics). ..

Article Title: GLUT1 gene is a potential hypoxic marker in colorectal cancer patients
Article Snippet: .. The lifts were covered with Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA) containing DIG-11-UTP-labeled cDNA probes. .. After the washing and blocking steps, the arrays were incubated with alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Diagnostics).

Article Title: Significance of the glycolytic pathway and glycolysis related-genes in tumorigenesis of human colorectal cancers
Article Snippet: .. The lifts were covered with Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Diagnostics GmbH) and blocking. ..

Article Title: Great potential of a panel of multiple hMTH1, SPD, ITGA11 and COL11A1 markers for diagnosis of patients with non-small cell lung cancer
Article Snippet: .. The lifts were covered with the Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA, USA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphataseconjugated anti-digoxigenin antibody (Roche Diagnostics). ..

Mutagenesis:

Article Title: The Bax/Bak ortholog in Drosophila , Debcl, exerts limited control over programmed cell death
Article Snippet: .. The membranes were probed with [ 32 P]dCTP-labeled DNA from PCR product using primers 5′-GCT ACA GTC GAG TGT GCT GGG TTG TTT GCG-3′ and 5′-GAC GGC GGA TTC CAG ACG CTT TCA GAA CGG-3′ for verification of the left recombination arm, and primers 5′-GCG TAC AAT TAG ACC AGC CGT TGT GTT GGC-3′ and 5′-AAG AGG ACA ACA GCG AGG TGG AGG AGG ACG-3′ for verification of the right recombination arm, and hybridized using Express Hyb Hybridization Solution (BD Biosciences). fig ft0 fig mode=article f1 Fig. 1. caption a4 Generation and verification of a targeted debcl mutation. ( A ) Targeting scheme for the debcl gene. .. Southern blotting For verification of targeting, genomic DNA was prepared from flies carrying the candidate targeted allele using the Wizard Genomic Purification Kit (Promega).

Incubation:

Article Title: Simultaneous detection of multiple mRNA markers CK19, CEA, c-Met, Her2/neu and hMAM with membrane array, an innovative technique with a great potential for breast cancer diagnosis.
Article Snippet: The objective of this study was mainly to develop and evaluate a membrane array-based method simultaneously detecting the expression levels of a multiple mRNAmarker panel in the peripheral blood for used in complementary breast cancer diagnosis.. The mRNA markers employed included cytokeratin 19 (CK-19), carcinoembryonic antigen (CEA), c-Met, Her2/neu, and mammaglobin (hMAM).. The specimens of peripheral blood were collected from 80 healthy women and 102 female patients with breast cancer.

Article Title: Overexpression of EGFR pathway-related genes in the circulation is highly correlated with EGFR mutations and overexpression in paired cancer tissue from patients with non-small cell lung cancer
Article Snippet: .. The lifts were covered with the Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA, USA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphatase-conjugated antidigoxigenin antibody (Roche Diagnostics). ..

Article Title: Significance of the glycolytic pathway and glycolysis related-genes in tumorigenesis of human colorectal cancers
Article Snippet: .. The lifts were covered with Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Diagnostics GmbH) and blocking. ..

Article Title: Great potential of a panel of multiple hMTH1, SPD, ITGA11 and COL11A1 markers for diagnosis of patients with non-small cell lung cancer
Article Snippet: .. The lifts were covered with the Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA, USA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphataseconjugated anti-digoxigenin antibody (Roche Diagnostics). ..

Blocking Assay:

Article Title: Significance of the glycolytic pathway and glycolysis related-genes in tumorigenesis of human colorectal cancers
Article Snippet: .. The lifts were covered with Express Hyb Hybridization Solution (BD Biosciences, Palo Alto, CA) containing DIG-11-UTP-labeled cDNA probes, and then incubated with alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Diagnostics GmbH) and blocking. ..



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